6K26

Crystal structure of Vibrio cholerae methionine aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-06-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 1 21 1
Unit cell lengths	49.529, 50.010, 131.086
Unit cell angles	90.00, 97.25, 90.00

Refinement procedure

Resolution	34.660 - 1.850
R-factor	0.1679
Rwork	0.166
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mat
RMSD bond length	0.011
RMSD bond angle	1.626
Data reduction software	HKL-2000 (v716)
Data scaling software	HKL-2000 (v716)
Phasing software	MOLREP (11.6.03)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.083	0.042	0.531
Rmeas	0.101	0.051	0.648
Rpim	0.057	0.029	0.366
Total number of observations	155933
Number of reflections	53682	5567	5260
<I/σ(I)>	8.9
Completeness [%]	98.5	98.7	97.8
Redundancy	2.9	2.9	2.8
CC(1/2)		0.997	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 12 % PEG 3350