6K25

Crystal structure of Ca-unbound human Annexin A5 in low salt condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193.15
Detector technology	CCD
Collection date	2018-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 63 2 2
Unit cell lengths	94.512, 94.512, 136.701
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.870 - 2.401
R-factor	0.2302
Rwork	0.227
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xo3
RMSD bond length	0.001
RMSD bond angle	0.399
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.083	0.385
Rpim	0.017	0.150
Number of reflections	13961	658
<I/σ(I)>	21.2
Completeness [%]	94.8
Redundancy	15.7
CC(1/2)		0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.2 M Sodium phosphate dibasic dehydrate 20% w/v Polyethylene glycol 3,350 2 mM TCEP