6K25
Crystal structure of Ca-unbound human Annexin A5 in low salt condition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 193.15 |
Detector technology | CCD |
Collection date | 2018-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 94.512, 94.512, 136.701 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.870 - 2.401 |
R-factor | 0.2302 |
Rwork | 0.227 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xo3 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.399 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.083 | 0.385 |
Rpim | 0.017 | 0.150 |
Number of reflections | 13961 | 658 |
<I/σ(I)> | 21.2 | |
Completeness [%] | 94.8 | |
Redundancy | 15.7 | |
CC(1/2) | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M Sodium phosphate dibasic dehydrate 20% w/v Polyethylene glycol 3,350 2 mM TCEP |