6K20
Structure of Apo YdiU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-03-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 42.526, 117.352, 147.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.891 - 2.580 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.666 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.15.1_3469: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 2.690 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 22642 | 2275 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 93.6 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 10% peg200, 0.1M Bistris pH9.0, 18%PEG 8,000 |
