6K1Y
Crystal structure of Rhodothermus marinus substrate-binding protein at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.544, 48.601, 57.821 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.200 - 1.900 |
R-factor | 0.1831 |
Rwork | 0.178 |
R-free | 0.22780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z6v |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.101 | 0.480 |
Rmeas | 0.107 | 0.543 |
Rpim | 0.034 | 0.245 |
Number of reflections | 10511 | 494 |
<I/σ(I)> | 74.57 | 4.096 |
Completeness [%] | 95.3 | 91.3 |
Redundancy | 7.6 | 4.2 |
CC(1/2) | 0.995 | 0.499 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | HEPES, PEG 3350 |