6K1X
Crystal structure of Rhodothermus marinus substrate-binding protein at pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 34.913, 35.725, 115.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.941 - 1.802 |
R-factor | 0.1935 |
Rwork | 0.190 |
R-free | 0.22040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z6v |
RMSD bond length | 0.007 |
RMSD bond angle | 0.809 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.874 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.118 | 0.425 |
Rmeas | 0.132 | 0.471 |
Rpim | 0.057 | 0.198 |
Number of reflections | 12659 | 1440 |
<I/σ(I)> | 27.6 | 6.2 |
Completeness [%] | 90.5 | 95 |
Redundancy | 4.8 | |
CC(1/2) | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | Ammonium sulfate, MES |