6K1E

Crystal structure of EXD2 exonuclease domain soaked in Mg and GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97936
Spacegroup name	P 21 21 21
Unit cell lengths	46.496, 73.288, 134.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.467 - 2.900
R-factor	0.23
Rwork	0.228
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k17
RMSD bond length	0.005
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.109	0.343
Number of reflections	10440	467
<I/σ(I)>	13.54
Completeness [%]	96.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Magnesium chloride, guanosine monophosphate