6K17

Crystal structure of EXD2 exonuclease domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97940
Spacegroup name	P 21 21 21
Unit cell lengths	45.955, 72.607, 135.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.332 - 1.602
R-factor	0.1928
Rwork	0.192
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbt
RMSD bond length	0.010
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.127	0.715
Number of reflections	60416	2976
<I/σ(I)>	22.54
Completeness [%]	99.5
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol