6K0S

Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with nigerose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	132.557, 132.557, 76.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.222 - 1.534
R-factor	0.1579
Rwork	0.157
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k0m
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.918	1.630
High resolution limit [Å]	1.530	1.530
Rmerge	0.143	1.277
Number of reflections	101377	31312
<I/σ(I)>	8.9
Completeness [%]	99.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5 M (NH4)2SO4, 25% (v/v) glycerol