6K0L
The crystal structure of simian CD163 SRCR5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.623, 33.465, 46.726 |
| Unit cell angles | 71.02, 75.29, 84.91 |
Refinement procedure
| Resolution | 31.660 - 1.580 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.169 |
| Data reduction software | xia2 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.040 | 0.096 |
| Number of reflections | 20604 | 2039 |
| <I/σ(I)> | 24.6 | |
| Completeness [%] | 95.1 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH5.5, 25% PEG 3350, 0.2M NaCl |
