6K0L
The crystal structure of simian CD163 SRCR5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-07-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 28.623, 33.465, 46.726 |
Unit cell angles | 71.02, 75.29, 84.91 |
Refinement procedure
Resolution | 31.660 - 1.580 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1by2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.169 |
Data reduction software | xia2 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.040 | 0.096 |
Number of reflections | 20604 | 2039 |
<I/σ(I)> | 24.6 | |
Completeness [%] | 95.1 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH5.5, 25% PEG 3350, 0.2M NaCl |