6K07

Crystal structure of REV7(R124A) in complex with a Shieldin3 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	61.080, 61.080, 133.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.160 - 2.240
R-factor	0.2062
Rwork	0.204
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.010
RMSD bond angle	1.059
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.160	2.300
High resolution limit [Å]	2.240	2.230
Rpim	0.021	0.505
Number of reflections	14467	13263
<I/σ(I)>	19.3	2.3
Completeness [%]	99.8
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Sodium acetate, Ammonium sulfate