6K01
Crystal structure of xH2A-H2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 |
| Unit cell lengths | 46.923, 46.923, 67.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.637 - 2.839 |
| R-factor | 0.2286 |
| Rwork | 0.223 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.640 | 2.940 |
| High resolution limit [Å] | 2.839 | 2.840 |
| Rmerge | 0.831 | |
| Number of reflections | 3756 | 3756 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Ammonium sulfate, 0.1M HEPES pH=7.5, 25% PEG 3350 |
