6JZU
The crystal structure of acyl-acyl carrier protein (acyl-ACP) reductase (AAR) in complex with aldehyde deformylating oxygenase (ADO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.377, 71.313, 137.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.427 - 2.181 |
R-factor | 0.2064 |
Rwork | 0.203 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rc8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.857 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.430 | 2.260 |
High resolution limit [Å] | 2.180 | 2.180 |
Rmerge | 0.086 | 1.090 |
Rpim | 0.025 | 0.310 |
Number of reflections | 34553 | 1708 |
<I/σ(I)> | 30 | |
Completeness [%] | 99.6 | |
Redundancy | 13.1 | |
CC(1/2) | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 0.1M HEPES, pH 7.5, 20% PEG 8000 |