6JZP
Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xylotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.236, 59.513, 70.189 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.753 - 1.120 |
R-factor | 0.1281 |
Rwork | 0.128 |
R-free | 0.14050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dfc |
RMSD bond length | 0.005 |
RMSD bond angle | 0.902 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.753 | 1.160 |
High resolution limit [Å] | 1.120 | 1.120 |
Number of reflections | 71926 | 5237 |
<I/σ(I)> | 15.26 | 1.68 |
Completeness [%] | 91.2 | 67.79 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 291 | 20% PEG 8000, 0.2M NaI, 0.1M MES |