6JZK

Structure of FimA type-1 (FimA1) prepilin of the type V major fimbrium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	35.840, 85.452, 242.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.726 - 2.100
R-factor	0.1915
Rwork	0.189
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jzj
RMSD bond length	0.007
RMSD bond angle	0.818
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.050	0.397
Rmeas	0.059	0.468
Rpim	0.031	0.244
Number of reflections	44884	3585
<I/σ(I)>	21.3	4.4
Completeness [%]	100.0	100
Redundancy	6.6	6.9
CC(1/2)	0.999	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1M Sodium citrate pH5.6, 1.8M ammonium sulfate, 0.2M sodium potassium tartrate