6JYW
Crystal structure of the transcription regulator CadR N81M mutant from P. putida in complex with Cadmium(II) and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 126.435, 126.435, 93.783 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.950 |
R-factor | 0.20504 |
Rwork | 0.202 |
R-free | 0.25293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jgx |
RMSD bond length | 0.009 |
RMSD bond angle | 1.383 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.800 | |
Number of reflections | 17117 | |
<I/σ(I)> | 20.1 | 2.4 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | HEPES, sodium citrate tribasic dihydrate |