6JYU

Crystal structure of Human G6PD Canton

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-25
Detector	RAYONIX MX300-HS
Wavelength(s)	0.98
Spacegroup name	F 2 2 2
Unit cell lengths	60.422, 172.830, 215.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.210 - 1.890
R-factor	0.1557
Rwork	0.154
R-free	0.19190
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.210	1.970
High resolution limit [Å]	1.890	1.900
Number of reflections	45396
<I/σ(I)>	34.25
Completeness [%]	99.2
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Tris, 20% glycerol ethoxylate, 10% tetrahydrofuran