6JYS
GII.13/21 noroviruses recognize glycans with a terminal beta-galactose via an unconventional glycan binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 113 |
Detector technology | PIXEL |
Collection date | 2018-06-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97917 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.728, 99.695, 221.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.635 - 1.697 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.697 | 1.700 |
Number of reflections | 144550 | |
<I/σ(I)> | 19.652 | |
Completeness [%] | 96.8 | |
Redundancy | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 5% polyethylene glycol 3350 |