6JYI
Crystal structure of the PadR-like transcriptional regulator BC1756 from Bacillus cereus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-03 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97939 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.682, 132.672, 49.249 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.920 |
R-factor | 0.20884 |
Rwork | 0.207 |
R-free | 0.24672 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.418 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.060 | 0.471 |
Number of reflections | 31084 | 3033 |
<I/σ(I)> | 43.9 | 3.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | PEG 3350, sodium citrate, ammonium dihydrogen phosphate |