6JYF
Structure of light-state marine bacterial chloride importer, NM-R3, with Pulse laser (ND-1%) at 140K.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW14A |
Synchrotron site | Photon Factory |
Beamline | AR-NW14A |
Temperature [K] | 140 |
Detector technology | CCD |
Collection date | 2018-11-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.918, 49.289, 69.466 |
Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
Resolution | 34.072 - 2.004 |
R-factor | 0.1641 |
Rwork | 0.162 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ztk |
RMSD bond length | 0.003 |
RMSD bond angle | 0.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.100 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 21264 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 95.9 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 30% polyethylene glycol dimethyl ether 500, 0.15 M sodium chloride, 0.15M calcium chloride, and 0.1 M MES (pH 6.0) buffer |