6JYA
Structure of dark-state marine bacterial chloride importer, NM-R3, with CW laser (ND-10%) at 95K.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.671, 49.056, 69.228 |
Unit cell angles | 90.00, 109.99, 90.00 |
Refinement procedure
Resolution | 32.529 - 1.803 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ztk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.826 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.529 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 29435 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 91.5 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 30% polyethylene glycol dimethyl ether 500, 0.15 M sodium chloride, 0.15M calcium chloride, and 0.1 M MES (pH 6.0) buffer |