6JY8
Structure of dark-state marine bacterial chloride importer, NM-R3, with CW laser (ND-3%) at 95K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-11-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.603, 49.148, 69.186 |
| Unit cell angles | 90.00, 110.06, 90.00 |
Refinement procedure
| Resolution | 33.943 - 1.900 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ztk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.943 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25074 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 97.5 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 30% polyethylene glycol dimethyl ether 500, 0.15 M sodium chloride, 0.15M calcium chloride, and 0.1 M MES (pH 6.0) buffer |
