6JY6

Structure of dark-state marine bacterial chloride importer, NM-R3, with Pulse laser (ND-1%) at 95K.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	102.584, 48.930, 69.170
Unit cell angles	90.00, 110.03, 90.00

Refinement procedure

Resolution	33.875 - 1.800
R-factor	0.1689
Rwork	0.167
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ztk
RMSD bond length	0.004
RMSD bond angle	0.826
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.900	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	28518
<I/σ(I)>	18.5
Completeness [%]	95.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	30% polyethylene glycol dimethyl ether 500, 0.15 M sodium chloride, 0.15M calcium chloride, and 0.1 M MES (pH 6.0) buffer