6JXL

Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xylotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	280
Detector technology	CCD
Collection date	2018-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.540, 58.969, 69.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.670 - 1.300
R-factor	0.153
Rwork	0.152
R-free	0.16770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dfc
RMSD bond length	0.014
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.965	1.347
High resolution limit [Å]	1.300	1.300
Number of reflections	39057	1793
<I/σ(I)>	14.7	1.7
Completeness [%]	80.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	280	20%PEG 8000, 0.2M NaI,0.1M MES