6JXG
Crystasl Structure of Beta-glucosidase D2-BGL from Chaetomella Raphigera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-05-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.774, 77.661, 123.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.190 - 1.900 |
R-factor | 0.14726 |
Rwork | 0.145 |
R-free | 0.19495 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iib |
RMSD bond length | 0.020 |
RMSD bond angle | 1.996 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.090 | 0.475 |
Number of reflections | 49111 | 4700 |
<I/σ(I)> | 27.7 | |
Completeness [%] | 93.8 | 91.5 |
Redundancy | 8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 10% (w/v) PEG 3000, 0.2 M magnesium chloride, 0.1 M sodium cacodylate, pH 6.5. |