6JXG
Crystasl Structure of Beta-glucosidase D2-BGL from Chaetomella Raphigera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-05-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.774, 77.661, 123.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.190 - 1.900 |
| R-factor | 0.14726 |
| Rwork | 0.145 |
| R-free | 0.19495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iib |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.475 |
| Number of reflections | 49111 | 4700 |
| <I/σ(I)> | 27.7 | |
| Completeness [%] | 93.8 | 91.5 |
| Redundancy | 8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 10% (w/v) PEG 3000, 0.2 M magnesium chloride, 0.1 M sodium cacodylate, pH 6.5. |
