6JX6

Tetrameric form of Smac

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	296
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	82.195, 85.403, 114.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.600 - 2.805
R-factor	0.2186
Rwork	0.216
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g73
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.905
High resolution limit [Å]	2.800	2.805
Rpim	0.045	0.202
Number of reflections	19689	1880
<I/σ(I)>	22.5
Completeness [%]	96.6
Redundancy	10.5
CC(1/2)	0.996	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		296	200mM Potassium Phosphate Monobasic, 20% PEG 3350