6JX1
Crystal structure of Formate dehydrogenase mutant V198I/C256I/P260S/E261P/S381N/S383F from Pseudomonas sp. 101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-28 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.496, 53.910, 117.063 |
Unit cell angles | 90.00, 121.37, 90.00 |
Refinement procedure
Resolution | 42.772 - 2.233 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2go1 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.852 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.230 | 0.530 |
Number of reflections | 27557 | 1285 |
<I/σ(I)> | 4.33 | 1.68 |
Completeness [%] | 74.6 | 35.56 |
Redundancy | 2.7 | 2.6 |
CC(1/2) | 0.879 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% w/v Polyethylene glycol 8,000. |