6JW5
RVD Q* recognizes 5hmC through water-mediated H bonds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-10 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.415, 93.777, 168.163 |
| Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
| Resolution | 164.030 - 2.990 |
| R-factor | 0.2448 |
| Rwork | 0.244 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gjp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.465 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 164.030 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmeas | 0.100 | 0.100 |
| Number of reflections | 46633 | 46633 |
| <I/σ(I)> | 1.52 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8-10% PEG3350 (w/v), 10% ethanol and 0.1M MES pH 6.7 |
