6JW3
Degenerate RVD RG forms a distinct loop conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.169, 93.622, 167.632 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.24442 |
| Rwork | 0.243 |
| R-free | 0.27782 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gjp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmeas | 0.092 | 0.092 |
| Number of reflections | 73997 | 43876 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8-10% PEG3350 (w/v), 10% ethanol and 0.1M MES pH 6.7 |
