6JW2
Universal RVD R* accommodates 5hmC via water-mediated interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-03 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.517, 93.776, 167.749 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 163.670 - 3.030 |
| R-factor | 0.2385 |
| Rwork | 0.237 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gjp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.461 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 163.670 | 3.110 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmeas | 0.118 | 0.118 |
| Number of reflections | 44929 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8-10% PEG3350 (w/v), 10% ethanol and 0.1M MES pH 6.7 |
