6JW0
Universal RVD R* accommodates cytosine via water-mediated interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-20 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.836, 87.396, 88.135 |
| Unit cell angles | 90.00, 104.12, 90.00 |
Refinement procedure
| Resolution | 85.470 - 2.200 |
| R-factor | 0.2242 |
| Rwork | 0.222 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gjp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.470 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.077 | 0.077 |
| Number of reflections | 58923 | |
| <I/σ(I)> | 3.45 | |
| Completeness [%] | 96.8 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8-10% PEG3350 (w/v), 10% ethanol and 0.1M MES pH 6.7 |
