6JV8

Crystal structure of Rat C5a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-25
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P -1
Unit cell lengths	26.250, 36.870, 41.490
Unit cell angles	90.39, 105.09, 95.77

Refinement procedure

Resolution	25.202 - 1.582
R-factor	0.2314
Rwork	0.229
R-free	0.27360
RMSD bond length	0.006
RMSD bond angle	0.730
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.582	1.582
Number of reflections	13647
<I/σ(I)>	11
Completeness [%]	89.0
Redundancy	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.02M CaCl2, 0.02M CdCl2, 0.02M CoCl2, 20% w/v polyethylene glycol 3350