6JV1
Crystal Structure of N-terminal domain of ArgZ, C264S mutant, bound to Substrate, an arginine dihydrolase from the Ornithine-Ammonia Cycle in Cyanobacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.346, 87.256, 42.818 |
Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
Resolution | 43.630 - 1.200 |
R-factor | 0.1724 |
Rwork | 0.172 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jv0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.180 |
High resolution limit [Å] | 1.160 | 3.150 | 1.160 |
Rmerge | 0.093 | 0.081 | 0.747 |
Rmeas | 0.101 | 0.088 | 0.821 |
Rpim | 0.038 | 0.034 | 0.335 |
Number of reflections | 98683 | 5148 | 4750 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 97.0 | 99.1 | 93.7 |
Redundancy | 6.7 | 6.8 | 5.6 |
CC(1/2) | 0.989 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M KI, 0.1 M MES pH 6.5, 19% PEG 4000 |