6JV0
Crystal Structure of N-terminal domain of ArgZ, bound to Product, an arginine dihydrolase from the Ornithine-Ammonia Cycle in Cyanobacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.468, 87.746, 43.310 |
Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
Resolution | 43.870 - 1.140 |
R-factor | 0.1737 |
Rwork | 0.173 |
R-free | 0.19210 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMPLE |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.870 | 43.870 | 1.160 |
High resolution limit [Å] | 1.140 | 6.240 | 1.140 |
Rmerge | 0.073 | 0.083 | 0.545 |
Rmeas | 0.082 | 0.094 | 0.614 |
Rpim | 0.037 | 0.043 | 0.277 |
Total number of observations | 514396 | 3355 | 24720 |
Number of reflections | 108732 | 692 | 5319 |
<I/σ(I)> | 13.1 | 22.3 | 3 |
Completeness [%] | 99.4 | 99.2 | 99.2 |
Redundancy | 4.7 | 4.8 | 4.6 |
CC(1/2) | 0.993 | 0.976 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M KI, 0.1 M MES pH 6.5, 19% PEG 4000 |