6JV0

Crystal Structure of N-terminal domain of ArgZ, bound to Product, an arginine dihydrolase from the Ornithine-Ammonia Cycle in Cyanobacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978530
Spacegroup name	P 1 21 1
Unit cell lengths	40.468, 87.746, 43.310
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	43.870 - 1.140
R-factor	0.1737
Rwork	0.173
R-free	0.19210
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMPLE
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.870	43.870	1.160
High resolution limit [Å]	1.140	6.240	1.140
Rmerge	0.073	0.083	0.545
Rmeas	0.082	0.094	0.614
Rpim	0.037	0.043	0.277
Total number of observations	514396	3355	24720
Number of reflections	108732	692	5319
<I/σ(I)>	13.1	22.3	3
Completeness [%]	99.4	99.2	99.2
Redundancy	4.7	4.8	4.6
CC(1/2)	0.993	0.976	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.2 M KI, 0.1 M MES pH 6.5, 19% PEG 4000