6JUV
Crytsal structure of ScpB derived from Pyrococcus yayanosii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 92.300, 92.300, 111.817 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.300 - 3.043 |
R-factor | 0.2387 |
Rwork | 0.236 |
R-free | 0.26490 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.495 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.300 | 3.250 |
High resolution limit [Å] | 3.040 | 3.040 |
Rmerge | 0.171 | 0.386 |
Rmeas | 0.172 | 0.387 |
Rpim | 0.017 | 0.035 |
Number of reflections | 9802 | 1721 |
<I/σ(I)> | 34.3 | 20.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 113.1 | 118.9 |
CC(1/2) | 0.999 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 35% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 0.1M MES (pH 5.5), 0.4M sodium chloride |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 35% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 0.1M MES (pH 5.5), 0.4M sodium chloride |