6JUS
MsDpo4-DNA complex 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.730, 80.340, 208.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 2.500 |
| R-factor | 0.20794 |
| Rwork | 0.206 |
| R-free | 0.25425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jul |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.233 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.552 |
| Rpim | 0.042 | 0.268 |
| Number of reflections | 31909 | 4401 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.0 | 95.3 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2KMME 12%, Bis Tris Propane pH 6.0, NaCl 0.2 mM |
