6JUR
MsDpo4-DNA complex 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.953724 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.670, 80.240, 210.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.260 - 2.060 |
R-factor | 0.21122 |
Rwork | 0.209 |
R-free | 0.25733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jul |
RMSD bond length | 0.027 |
RMSD bond angle | 2.417 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.260 | 2.180 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.072 | 0.577 |
Rpim | 0.032 | 0.256 |
Number of reflections | 58048 | 8368 |
<I/σ(I)> | 13.7 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.1 | 6 |
CC(1/2) | 0.998 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2KMME 14%, NaCl 0.2mM, Bis Tris Propane pH 6.0 |