6JUP
Mutant PolIV-DNA incoming nucleotide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.184, 56.974, 111.136 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 69.710 - 2.440 |
| R-factor | 0.22227 |
| Rwork | 0.221 |
| R-free | 0.24505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4irc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.183 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.710 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.057 | 0.755 |
| Rpim | 0.313 | |
| Number of reflections | 40567 | 5837 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 6.6 | 6.7 |
| CC(1/2) | 0.999 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | MPD 2%, Sodium Phosphate buffer pH 4.5 |
