6JUP
Mutant PolIV-DNA incoming nucleotide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0723 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.184, 56.974, 111.136 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 69.710 - 2.440 |
R-factor | 0.22227 |
Rwork | 0.221 |
R-free | 0.24505 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4irc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.183 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.710 | 2.570 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.057 | 0.755 |
Rpim | 0.313 | |
Number of reflections | 40567 | 5837 |
<I/σ(I)> | 14.4 | |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 6.6 | 6.7 |
CC(1/2) | 0.999 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | MPD 2%, Sodium Phosphate buffer pH 4.5 |