6JUM
MsDpo4-DNA complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.020, 80.960, 211.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.960 - 1.780 |
| R-factor | 0.19724 |
| Rwork | 0.195 |
| R-free | 0.23377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jul |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.810 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.119 | |
| Number of reflections | 89850 | 12978 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 9.8 | 10 |
| CC(1/2) | 0.998 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2KMME 12%, NaCl 0.2 mM, Bis Tris Propane pH 6.0. |
