6JUM
MsDpo4-DNA complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.020, 80.960, 211.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.960 - 1.780 |
R-factor | 0.19724 |
Rwork | 0.195 |
R-free | 0.23377 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jul |
RMSD bond length | 0.026 |
RMSD bond angle | 2.245 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.810 | 1.880 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.119 | |
Number of reflections | 89850 | 12978 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 9.8 | 10 |
CC(1/2) | 0.998 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2KMME 12%, NaCl 0.2 mM, Bis Tris Propane pH 6.0. |