6JUI
The atypical Myb-like protein Cdc5 contains two distinct nucleic acid-binding surfaces
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.780, 76.277, 54.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.139 - 2.402 |
R-factor | 0.2378 |
Rwork | 0.234 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.140 | 2.488 |
High resolution limit [Å] | 2.402 | 2.402 |
Number of reflections | 11354 | |
<I/σ(I)> | 22.1 | |
Completeness [%] | 99.3 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 40% MPD |