6JUF
SspB crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.398, 81.637, 148.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.587 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.23840 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.810 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 1.840 |
High resolution limit [Å] | 1.587 | 1.750 |
Number of reflections | 93617 | 93617 |
<I/σ(I)> | 18 | |
Completeness [%] | 98.8 | |
Redundancy | 7.3 | |
CC(1/2) | 0.862 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% polyethyleneglycol (PEG) 3350, 0.2 M magnesium chloride |