6JU7
Aspergillus oryzae active-tyrosinase copper-depleted C92A mutant complexed with L-tyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.504, 104.896, 154.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.420 |
R-factor | 0.1554 |
Rwork | 0.151 |
R-free | 0.19450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w6w |
RMSD bond length | 0.006 |
RMSD bond angle | 0.020 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL (2017/1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.087 | 0.430 |
Number of reflections | 167149 | 7469 |
<I/σ(I)> | 20.2 | 4.5 |
Completeness [%] | 99.4 | 89.6 |
Redundancy | 6.6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 20% polyethylene glycol (PEG) 3350, 30mM NH4NO3 |