6JU5
Aspergillus oryzae pro-tyrosinase C92A/F513Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-24 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.344, 118.210, 79.363 |
Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.340 |
R-factor | 0.1593 |
Rwork | 0.157 |
R-free | 0.20220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w6w |
RMSD bond length | 0.006 |
RMSD bond angle | 0.020 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL (2017/1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.076 | 0.690 |
Number of reflections | 223551 | 11171 |
<I/σ(I)> | 17.3 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 19% polyethylene glycol (PEG) 3350, 30mM NH4NO3 |