6JU0
Mouse antibody 3.3 Fab in complex with PEG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.299, 177.347, 89.019 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 24.503 - 2.601 |
| R-factor | 0.2117 |
| Rwork | 0.211 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w9d |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.052 | 0.361 |
| Number of reflections | 64434 | 6420 |
| <I/σ(I)> | 15.1 | 2.2 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.928 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | PEG 4000, 2-propanol, PEG 2000 MME, Ni-citrate |
