6JTO

Crystal structure of HLA-C05 in complex with a tumor mut10m peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2017-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	69.360, 76.330, 84.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.678 - 1.700
R-factor	0.1799
Rwork	0.179
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.678	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.027	0.189
Number of reflections	49991	4913
<I/σ(I)>	10.84
Completeness [%]	100.0	100
Redundancy	2	2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2M ammonium acetate, 0.1M HEPES (pH 7.5), 25% w/v polyethylene glycol 3350