6JT6
Crystal structure of cytochrome b domain of Pyranose Dehydrogenase from Coprinopsis cinerea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.138, 59.741, 40.858 |
| Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
| Resolution | 39.140 - 2.000 |
| R-factor | 0.1569 |
| Rwork | 0.154 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d7d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.390 |
| Number of reflections | 12797 | 1999 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.996 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 293 | 40% MPD, 5mM Tris-HCl pH8.0 |
