6JT5
Crystal structure of PQQ doamin of Pyranose Dehydrogenase from Coprinopsis cinerea: apo-from
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-10-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.298, 47.851, 69.375 |
Unit cell angles | 90.00, 115.55, 90.00 |
Refinement procedure
Resolution | 19.750 - 1.500 |
R-factor | 0.107 |
Rwork | 0.105 |
R-free | 0.13400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.081 | |
Number of reflections | 60275 | |
<I/σ(I)> | 32.9 | |
Completeness [%] | 99.4 | 98.3 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 5mM sodium acetate, 0.2M sodium formate, 20% PEG 3350, 20mM calcium chloride |