6JSB
Crystal structure of Heme binding protein HtaB from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.170, 33.730, 126.620 |
| Unit cell angles | 90.00, 121.14, 90.00 |
Refinement procedure
| Resolution | 38.091 - 1.699 |
| R-factor | 0.1778 |
| Rwork | 0.177 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6DSA |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.091 | 1.760 |
| High resolution limit [Å] | 1.699 | 1.700 |
| Rmerge | 0.073 | 0.563 |
| Rmeas | 0.086 | 0.660 |
| Rpim | 0.045 | 0.341 |
| Number of reflections | 94191 | 9232 |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.997 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 38 % 2-ethoxyethanol, 0.1 M Tris-HCl pH7.5, 0.05 M calcium acetate |
