6JSA

Crystal structure of the C-terminal domain of HtaA from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-13
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	C 2 2 21
Unit cell lengths	60.280, 63.310, 88.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.031 - 1.300
R-factor	0.1617
Rwork	0.160
R-free	0.18010
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.022
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.031	1.347
High resolution limit [Å]	1.300	1.300
Rmerge	0.098	0.529
Rmeas	0.106	0.570
Rpim	0.039	0.209
Number of reflections	41800	4125
<I/σ(I)>	12.14
Completeness [%]	99.9	99.64
Redundancy	7.3	7.3
CC(1/2)	0.997	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	3.0 M sodium acetate