6JRL
Crystal structure of Drosophila alpha methyldopa-resistant protein/3,4-dihydroxyphenylacetaldehyde synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.469, 117.307, 139.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.180 - 2.200 |
R-factor | 0.22028 |
Rwork | 0.218 |
R-free | 0.26708 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k40 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.306 |
Data reduction software | HKL-2000 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | 0.577 |
Number of reflections | 30527 | 3001 |
<I/σ(I)> | 32.9 | |
Completeness [%] | 100.0 | |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.16M MgCl, 0.085M NaCl, pH 6.5, 25.5% w/v PEG 8000, 15% glycerol |