6JRG

Crystal structure of ZmMoc1 H253A mutant in complex with Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	53.572, 77.841, 63.854
Unit cell angles	90.00, 97.93, 90.00

Refinement procedure

Resolution	34.250 - 2.005
R-factor	0.1878
Rwork	0.186
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6is9
RMSD bond length	0.007
RMSD bond angle	0.836
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.078	0.053	0.492
Rmeas	0.084	0.058	0.550
Rpim	0.033	0.023	0.242
Number of reflections	34594	1801	1663
<I/σ(I)>	7.7
Completeness [%]	99.2	99.5	96.4
Redundancy	6.4	6.3	4.6
CC(1/2)		0.995	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	0.1 M HEPES pH 7.5, 37.5% Polyethylene glycol 400